5 Tips about attune flow cytometer You Can Use Today
5 Tips about attune flow cytometer You Can Use Today
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Info-enriched characterization of mobile populations. Morphological information from illustrations or photos can insert towards the richness of flow cytometry information. For instance, the figure demonstrates an normally typical apoptosis assay employing Annexin V and PI, incorporating mobile imaging to characterize cells in Every single inhabitants to expose morphologically distinctive attributes. These insights couldn't are actually gained from multiplex staining by yourself.
Whether or not you’re a starter or a specialist, examine our assets to progress your exploration and know-how in flow cytometry.
Mix acoustic concentrating technologies with high-parameter Investigation for versatile spectral unmixing and conventional workflows.
The award-winning Invitrogen Attune CytPix Flow Cytometer combines acoustic concentrating using a superior-pace brightfield camera for simultaneous substantial throughput flow cytometry and large resolution brightfield imaging.
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Right here, a highly trained person has gated singlets confidently. Soon after evaluating the handbook singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate contains over 4% aggregates.
Optimizing gating technique. Even robust handbook singlet gating is error-inclined and remains a subjective selection stage in Practically all flow cytometry assays. Imaging can be employed to substantiate and alter gates to include only single cells of desire.
Reliable image top quality even at high throughput Acoustic concentrating and a high-pace digicam Incorporate to image these T cells continually at lower or high flow costs. Simply regulate target and digicam options to satisfy experimental prerequisites.
Early signs of this contamination had initially been dismissed as debris. Backgating to discover new populations. Backgating imaged cells about the Attune CytPix Flow Cytometer also permits you to use morphological attributes to find attention-grabbing subpopulations that would not be evident from flow cytometry details alone.
Consumers can view event visuals in real-time to verify sample high quality, optimize protocols, and record the morphology of every mobile populace identified for analysis. Imaging seize also Gains from acoustic focusing, which helps you to place the cells to ensure that a sharp, centered picture is acquired.
Audio waves permit exact mobile alignment, causing get more info exceptional facts excellent and ~10X a lot quicker sample flow fees.
Application Observe: Automatic impression analysis lowers user-to-consumer variability in flow cytometry gating tactics
Crafted-in dimensionality reduction applications greater resolution into populations. Fluorescently gated populations back-gated on to UMAP plot to substantiate expected population resolution in lysed total human blood. Person can look at functions from Every single cluster for Visible confirmation of cell phenotypes.
Versatile payment approach from Agilent Economic Options permit you to get the important instrumentation you would like for quick undertaking, or to increase your lab.
Gating on these extended parameters permits you to rapidly and properly identify populations of curiosity and ensure gating approach with little or no guide review. The Attune application provides access to point out-of-the-artwork device Studying Examination resources in an expertly made person interface, rendering it extremely simple to operate.
Because the cell line had Earlier been analyzed over the Attune CytPix Flow Cytometer, the researchers went back again to the images and had been able to doc the microbial infection a minimum of five days before.
The Attune CytPix Flow Cytometer will allow people to visualise samples throughout acquisition, confirm the precision of gating tactics, and guidance results with reputable documentation for publication applications.
What appeared to be a homogeneous inhabitants is definitely additional varied—an insight that needs to be thought of when interpreting results.